Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy.

Détails

ID Serval
serval:BIB_B18D1C7F85BB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy.
Périodique
Optics letters
Auteur(s)
Marquet P., Rappaz B., Magistretti P.J., Cuche E., Emery Y., Colomb T., Depeursinge C.
ISSN
0146-9592
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
30
Numéro
5
Pages
468-70
Langue
anglais
Notes
Publication types: Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.
Mots-clé
Algorithms, Animals, Cells, Cultured, Cerebral Cortex, Holography, Image Enhancement, Image Interpretation, Computer-Assisted, Mice, Microscopy, Phase-Contrast, Neurons, Signal Processing, Computer-Assisted
Pubmed
Web of science
Création de la notice
10/03/2008 10:58
Dernière modification de la notice
20/08/2019 15:20
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