In Search of Functional Advantages of Knots in Proteins.

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Ressource 1Download: journal.pone.0165986.PDF (4035.92 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_B15CF13C8D83
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In Search of Functional Advantages of Knots in Proteins.
Journal
PLoS One
Author(s)
Dabrowski-Tumanski P., Stasiak A., Sulkowska J.I.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
11
Number
11
Pages
e0165986
Language
english
Abstract
We analysed the structure of deeply knotted proteins representing three unrelated families of knotted proteins. We looked at the correlation between positions of knotted cores in these proteins and such local structural characteristics as the number of intra-chain contacts, structural stability and solvent accessibility. We observed that the knotted cores and especially their borders showed strong enrichment in the number of contacts. These regions showed also increased thermal stability, whereas their solvent accessibility was decreased. Interestingly, the active sites within these knotted proteins preferentially located in the regions with increased number of contacts that also have increased thermal stability and decreased solvent accessibility. Our results suggest that knotting of polypeptide chains provides a favourable environment for the active sites observed in knotted proteins. Some knotted proteins have homologues without a knot. Interestingly, these unknotted homologues form local entanglements that retain structural characteristics of the knotted cores.

Pubmed
Web of science
Open Access
Yes
Create date
28/11/2016 12:56
Last modification date
20/08/2019 15:20
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