Comparative Genomics Analysis of Streptococcus tigurinus Strains Identifies Genetic Elements Specifically and Uniquely Present in Highly Virulent Strains.

Détails

Ressource 1Télécharger: BIB_B14812A32BFE.P001.pdf (1110.26 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_B14812A32BFE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparative Genomics Analysis of Streptococcus tigurinus Strains Identifies Genetic Elements Specifically and Uniquely Present in Highly Virulent Strains.
Périodique
PloS one
Auteur(s)
Diene S.M., François P., Zbinden A., Entenza J.M., Resch G.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
11
Numéro
8
Pages
e0160554
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: epublish
Résumé
Streptococcus tigurinus is responsible for severe invasive infections such as infective endocarditis, spondylodiscitis and meningitis. As described, S. tigurinus isolates AZ_3aT and AZ_14 were highly virulent (HV phenotype) in an experimental model of infective endocarditis and showed enhanced adherence and invasion of human endothelial cells when compared to low virulent S. tigurinus isolate AZ_8 (LV phenotype). Here, we sought whether genetic determinants could explain the higher virulence of AZ_3aT and AZ_14 isolates. Several genetic determinants specific to the HV strains were identified through extensive comparative genomics amongst which some were thought to be highly relevant for the observed HV phenotype. These included i) an iron uptake and metabolism operon, ii) an ascorbate assimilation operon, iii) a newly acquired PI-2-like pilus islets described for the first time in S. tigurinus, iv) a hyaluronate metabolism operon, v) an Entner-Doudoroff pathway of carbohydrates metabolism, and vi) an alternate pathways for indole biosynthesis. We believe that the identified genomic features could largely explain the phenotype of high infectivity of the two HV S. tigurinus strains. Indeed, these features include determinants that could be involved at different stages of the disease such as survival of S. tigurinus in blood (iron uptake and ascorbate metabolism operons), initial attachment of bacterial pathogen to the damaged cardiac tissue and/or vegetation that formed on site (PI-2-like pilus islets), tissue invasion (hyaluronate operon and Entner-Doudoroff pathway) and regulation of pathogenicity (indole biosynthesis pathway).

Mots-clé
Ascorbic Acid/metabolism, Bacterial Adhesion/genetics, Biological Transport/genetics, Genomics, Hyaluronic Acid/metabolism, Indoles/metabolism, Iron/metabolism, Molecular Sequence Annotation, Phenotype, Proteomics, Species Specificity, Streptococcus/genetics, Streptococcus/metabolism, Streptococcus/pathogenicity, Tryptophan/metabolism, Virulence/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
30/09/2016 8:03
Dernière modification de la notice
08/05/2019 23:52
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