Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.

Détails

Ressource 1Télécharger: BIB_AF335B6D869D.P001.pdf (1933.50 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_AF335B6D869D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Generation of TALEN-mediated GRdim knock-in rats by homologous recombination.
Périodique
Plos One
Auteur(s)
Ponce de León V., Mérillat A.M., Tesson L., Anegón I., Hummler E.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
02/2014
Peer-reviewed
Oui
Volume
9
Numéro
2
Pages
e88146
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: epublish
Résumé
Transcription Activator-Like Effector Nucleases (TALEN) are potential tools for precise genome engineering of laboratory animals. We report the first targeted genomic integration in the rat using TALENs (Transcription Activator-Like Effector Nucleases) by homology-derived recombination (HDR). We assembled TALENs and designed a linear donor insert targeting a pA476T mutation in the rat Glucocorticoid Receptor (Nr3c1) namely GR(dim), that prevents receptor homodimerization in the mouse. TALEN mRNA and linear double-stranded donor were microinjected into rat one-cell embryos. Overall, we observed targeted genomic modifications in 17% of the offspring, indicating high TALEN cutting efficiency in rat zygotes.
Pubmed
Open Access
Oui
Création de la notice
05/09/2014 16:57
Dernière modification de la notice
20/08/2019 16:18
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