Identification of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections.

Détails

ID Serval
serval:BIB_AF226AEC1E2D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of a potent MAR element from the mouse genome and assessment of its activity in stable and transient transfections.
Périodique
Journal of Biotechnology
Auteur(s)
Harraghy N., Regamey A., Girod P.A., Mermod N.
ISSN
1873-4863 (Electronic)
ISSN-L
0168-1656
Statut éditorial
Publié
Date de publication
2011
Volume
154
Numéro
1
Pages
11-20
Langue
anglais
Résumé
Matrix attachment regions are DNA sequences found throughout eukaryotic genomes that are believed to define boundaries interfacing heterochromatin and euchromatin domains, thereby acting as epigenetic regulators. When included in expression vectors, MARs can improve and sustain transgene expression, and a search for more potent novel elements is therefore actively pursued to further improve recombinant protein production. Here we describe the isolation of new MARs from the mouse genome using a modified in silico analysis. One of these MARs was found to be a powerful activator of transgene expression in stable transfections. Interestingly, this MAR also increased GFP and/or immunoglobulin expression from some but not all expression vectors in transient transfections. This effect was attributed to the presence or absence of elements on the vector backbone, providing an explanation for earlier discrepancies as to the ability of this class of elements to affect transgene expression under such conditions.
Mots-clé
Animals, CHO Cells, Cricetinae, Cricetulus, Fluorescence, Gene Dosage/genetics, Gene Expression, Genetic Vectors/genetics, Genome/genetics, Green Fluorescent Proteins/metabolism, Hela Cells, Humans, Immunoglobulin G/biosynthesis, Matrix Attachment Regions/genetics, Mice, RNA, Messenger/genetics, RNA, Messenger/metabolism, Recombinant Proteins/metabolism, Transfection/methods, Transgenes/genetics
Pubmed
Web of science
Création de la notice
02/09/2011 9:48
Dernière modification de la notice
20/08/2019 15:18
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