Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter.

Détails

ID Serval
serval:BIB_AF078859333C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Feedback control of DnaA-mediated replication initiation by replisome-associated HdaA protein in Caulobacter.
Périodique
Journal of bacteriology
Auteur(s)
Collier J., Shapiro L.
ISSN
1098-5530[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
191
Numéro
18
Pages
5706-5716
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Chromosome replication in Caulobacter crescentus is tightly regulated to ensure that initiation occurs at the right time and only once during the cell cycle. The timing of replication initiation is controlled by both CtrA and DnaA. CtrA binds to and silences the origin. Upon the clearance of CtrA from the cell, the DnaA protein accumulates and allows loading of the replisome at the origin. Here, we identify an additional layer of replication initiation control that is mediated by the HdaA protein. In Escherichia coli, the Hda protein inactivates DnaA after replication initiation. We show that the Caulobacter HdaA homologue is necessary to restrict the initiation of DNA replication to only once per cell cycle and that it dynamically colocalizes with the replisome throughout the cell cycle. Moreover, the transcription of hdaA is directly activated by DnaA, providing a robust feedback regulatory mechanism that adjusts the levels of HdaA to inactivate DnaA.
Pubmed
Web of science
Open Access
Oui
Création de la notice
31/08/2009 14:52
Dernière modification de la notice
08/05/2019 23:45
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