Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms.
Details
Serval ID
serval:BIB_AEEC348DD0E9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms.
Journal
Applied Optics
ISSN
0003-6935 (Print)
ISSN-L
0003-6935
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
38
Number
34
Pages
6994-7001
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scalar diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system.
Pubmed
Web of science
Create date
05/04/2013 9:13
Last modification date
20/08/2019 15:18