Detecting N-RAS Q61R Mutated Thyroid Neoplasias by Immunohistochemistry.

Details

Serval ID
serval:BIB_AE8B53CB113E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detecting N-RAS Q61R Mutated Thyroid Neoplasias by Immunohistochemistry.
Journal
Endocrine pathology
Author(s)
Crescenzi A., Fulciniti F., Bongiovanni M., Giovanella L., Trimboli P.
ISSN
1559-0097 (Electronic)
ISSN-L
1046-3976
Publication state
Published
Issued date
03/2017
Peer-reviewed
Oui
Volume
28
Number
1
Pages
71-74
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Recently, the immunohistochemistry (IHC) for N-RAS Q61R has been developed and commercialized for clinical practice. Here, we investigated the reliability of IHC to identify N-RAS Q61R mutated thyroid neoplasia. A series of 24 consecutive thyroid lesions undergone surgery following indeterminate cytology were enrolled. Paraffin sections were stained for IHC using the rabbit monoclonal anti-human N-RAS Q61R, clone SP174. N-RAS mutations in codon 61 were also investigated by automated sequencing. At histology, 12 cases of follicular carcinoma, cytologically defined as follicular lesions, 1 papillary cancer, 7 follicular adenomas, and 4 hyperplastic nodules were found. Of these, 4 showed a positive IHC for anti N-RAS antibody where N-RAS expression was detected mainly at cytoplasmic level with similar intensity of reaction. The remaining cases had negative IHC. A 100% concordance between IHC and molecular analysis for N-RAS Q61R was observed. In conclusion, this study shows high reliability of IHC to identify N-RAS Q61R mutated thyroid lesions with high cost-effectiveness. These data indicate the reliability of IHC to identify N-RAS Q61R mutated thyroid neoplasia and suggest to adopt this approach for a more accurate management of patients, when indicated.

Keywords
Adult, Aged, DNA Mutational Analysis/methods, Female, GTP Phosphohydrolases/genetics, Humans, Immunohistochemistry/methods, Male, Membrane Proteins/genetics, Middle Aged, Reproducibility of Results, Thyroid Neoplasms/genetics, Thyroid Neoplasms/pathology
Pubmed
Web of science
Create date
09/01/2017 15:39
Last modification date
20/08/2019 15:18
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