Trafficking of Estrella lausannensis in human macrophages.

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Serval ID
serval:BIB_AE547AF449E3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Trafficking of Estrella lausannensis in human macrophages.
Journal
Pathogens and Disease
Author(s)
Rusconi B., Kebbi-Beghdadi C., Greub G.
ISSN
2049-632X (Electronic)
ISSN-L
2049-632X
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
73
Number
5
Pages
ftv027
Language
english
Abstract
Estrella lausannensis is a new member of the Chlamydiales order. Like other Chlamydia-related bacteria, it is able to replicate in amoebae and in fish cell lines. A preliminary study investigating the pathogenic potential of Chlamydia-related bacteria found a correlation between antibody response to E. lausannensis and pneumonia in children. To further investigate the pathogenic potential of E. lausannensis, we determined its ability to grow in human macrophages and its intracellular trafficking. The replication in macrophages resulted in viable E. lausannensis; however, it caused a significant cytopathic effect. The intracellular trafficking of E. lausannensis was analyzed by determining the interaction of the Estrella-containing inclusions with various endocytic markers as well as host organelles. The E. lausannensis inclusion escaped the endocytic pathway rapidly avoiding maturation into phagolysosomes by preventing both EEA-1 and LAMP-1 accumulation. Compared to Waddlia chondrophila, another Chlamydia-related bacteria, the recruitment of mitochondria and endoplasmic reticulum was minimal for E. lausannensis inclusions. Estrella lausannensis appears to use a distinct source of nutrients and energy compared to other members of the Chlamydiales order. In conclusion, we hypothesize that E. lausannensis has a restricted growth in human macrophages, due to its reduced capacity to control programmed cell death.
Keywords
Cell Line, Chlamydiales/growth & development, Chlamydiales/metabolism, Humans, Inclusion Bodies/microbiology, Macrophages/immunology, Macrophages/microbiology, Transport Vesicles/microbiology
Pubmed
Web of science
Open Access
Yes
Create date
04/02/2016 22:04
Last modification date
20/08/2019 15:18
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