PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds.

Détails

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_ACBD2CD355D9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
PHO1 Exports Phosphate from the Chalazal Seed Coat to the Embryo in Developing Arabidopsis Seeds.
Périodique
Current biology
Auteur(s)
Vogiatzaki E., Baroux C., Jung J.Y., Poirier Y.
ISSN
1879-0445 (Electronic)
ISSN-L
0960-9822
Statut éditorial
Publié
Date de publication
09/10/2017
Peer-reviewed
Oui
Volume
27
Numéro
19
Pages
2893-2900.e3
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Seed production requires the transfer of nutrients from the maternal seed coat to the filial endosperm and embryo. Because seed coat and filial tissues are symplasmically isolated, nutrients arriving in the seed coat via the phloem must be exported to the apoplast before reaching the embryo. Proteins implicated in the transfer of inorganic phosphate (Pi) from the seed coat to the embryo are unknown despite seed P content being an important agronomic trait. Here we show that the Arabidopsis Pi exporters PHO1 and PHOH1 are expressed in the chalazal seed coat (CZSC) of developing seeds. PHO1 is additionally expressed in developing ovules. Phosphorus (P) content and Pi flux between the seed coat and embryo were analyzed in seeds from grafts between WT roots and scions from either pho1, phoh1, or the pho1 phoh1 double mutant. Whereas P content and distribution between the seed coat and embryo in fully mature dry seeds of these mutants are similar to the WT, at the mature green stage of seed development the seed coat of the pho1 and pho1 phoh1 mutants, but not of the phoh1 mutant, retains approximately 2-fold more P than its WT control. Expression of PHO1 under a CZSC-specific promoter complemented the seed P distribution phenotype of the pho1 phoh1 double mutant. CZSC-specific down-expression of PHO1 also recapitulated the seed P distribution phenotype of pho1. Together, these experiments show that PHO1 expression in the CZSC is important for the transfer of P from the seed coat to the embryo in developing seeds.

Mots-clé
Arabidopsis, chalaza, embryo, nutrient, phosphate, seed coat
Pubmed
Web of science
Création de la notice
13/03/2018 15:27
Dernière modification de la notice
20/08/2019 15:16
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