Inference of chromosomal inversion dynamics from Pool-Seq data in natural and laboratory populations of Drosophila melanogaster.

Détails

Ressource 1Télécharger: 24372777_BIB_ACBBE0FC7660.pdf (657.46 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_ACBBE0FC7660
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inference of chromosomal inversion dynamics from Pool-Seq data in natural and laboratory populations of Drosophila melanogaster.
Périodique
Molecular Ecology
Auteur(s)
Kapun M., van Schalkwyk H., McAllister B., Flatt T., Schlötterer C.
ISSN
1365-294X (Electronic)
ISSN-L
0962-1083
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
23
Numéro
7
Pages
1813-1827
Langue
anglais
Résumé
Sequencing of pools of individuals (Pool-Seq) represents a reliable and cost-effective approach for estimating genome-wide SNP and transposable element insertion frequencies. However, Pool-Seq does not provide direct information on haplotypes so that, for example, obtaining inversion frequencies has not been possible until now. Here, we have developed a new set of diagnostic marker SNPs for seven cosmopolitan inversions in Drosophila melanogaster that can be used to infer inversion frequencies from Pool-Seq data. We applied our novel marker set to Pool-Seq data from an experimental evolution study and from North American and Australian latitudinal clines. In the experimental evolution data, we find evidence that positive selection has driven the frequencies of In(3R)C and In(3R)Mo to increase over time. In the clinal data, we confirm the existence of frequency clines for In(2L)t, In(3L)P and In(3R)Payne in both North America and Australia and detect a previously unknown latitudinal cline for In(3R)Mo in North America. The inversion markers developed here provide a versatile and robust tool for characterizing inversion frequencies and their dynamics in Pool-Seq data from diverse D. melanogaster populations.
Mots-clé
Animals, Australia, Chromosome Inversion, Drosophila melanogaster/genetics, Evolution, Molecular, Genetic Markers, Genetic Variation, Genetics, Population, Haplotypes, Karyotyping, Linkage Disequilibrium, North America, Polymorphism, Single Nucleotide, Sequence Analysis, DNA
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/01/2014 18:19
Dernière modification de la notice
20/08/2019 16:16
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