Article: article from journal or magazin.
Molecular cloning and expression of the human interferon-gamma receptor.
A cDNA encoding the human interferon-gamma receptor was isolated from a lambda gt11 expression library using a polyclonal antireceptor antiserum. The gene for this receptor was identified in a cosmid library and transfected into mouse cells. The human interferon-gamma receptor expressed in mouse cells displayed the same binding properties as in human cells. However, transfected cells were not sensitive to human IFN-gamma, suggesting the need for species-specific cofactors in receptor function. As inferred from the cDNA sequence, the human interferon-gamma receptor shows no similarities to known proteins and represents a novel transmembrane receptor. It is most likely the product of a single mRNA and a gene located on chromosome 6q.
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA/analysis, Gene Expression Regulation, Humans, Molecular Sequence Data, Molecular Weight, Receptors, Immunologic/genetics, Receptors, Interferon, Transfection
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