Molecular cloning and expression in Escherichia coli of bleomycin-resistance gene from a methicillin-resistant Staphylococcus aureus and its association with IS431 mec.
Details
Serval ID
serval:BIB_AB99EE013543
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular cloning and expression in Escherichia coli of bleomycin-resistance gene from a methicillin-resistant Staphylococcus aureus and its association with IS431 mec.
Journal
Applied microbiology and biotechnology
ISSN
0175-7598 (Print)
ISSN-L
0175-7598
Publication state
Published
Issued date
04/1995
Peer-reviewed
Oui
Volume
43
Number
1
Pages
65-69
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
A gene that confers bleomycin resistance was cloned from the chromosomal DNA of methicillin-resistant Staphylococcus aureus (MRSA) B-26 into the plasmid pUC18. It is of chromosomal origin rather than plasmid and exists in the chromosome making a cluster with the kanamycin-resistance gene. We found that the nucleotide sequence of the bleomycin-resistance gene from the chromosome of MRSA B-26 is identical to that from a staphylococcal plasmid, pUB110. The partial sequence of IS431mec was also found upstream from the DNA fragment containing the bleomycin- and kanamycin-resistance genes.
Keywords
Base Sequence, Bleomycin/pharmacology, Chromosomes, Bacterial, Cloning, Molecular, Drug Resistance, Microbial/genetics, Escherichia coli/genetics, Genes, Bacterial, Kanamycin Resistance/genetics, Methicillin Resistance, Molecular Sequence Data, Multigene Family, Staphylococcus aureus/genetics
Pubmed
Web of science
Create date
01/03/2018 16:43
Last modification date
27/09/2021 11:17
Usage data
