The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin.

Détails

ID Serval
serval:BIB_AB90922CC010
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The anti-influenza M2e antibody response is promoted by XCR1 targeting in pig skin.
Périodique
Scientific reports
Auteur(s)
Deloizy C., Fossum E., Barnier-Quer C., Urien C., Chrun T., Duval A., Codjovi M., Bouguyon E., Maisonnasse P., Hervé P.L., Barc C., Boulesteix O., Pezant J., Chevalier C., Collin N., Dalod M., Bogen B., Bertho N., Schwartz-Cornil I.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
09/08/2017
Peer-reviewed
Oui
Volume
7
Numéro
1
Pages
7639
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
XCR1 is selectively expressed on a conventional dendritic cell subset, the cDC1 subset, through phylogenetically distant species. The outcome of antigen-targeting to XCR1 may therefore be similar across species, permitting the translation of results from experimental models to human and veterinary applications. Here we evaluated in pigs the immunogenicity of bivalent protein structures made of XCL1 fused to the external portion of the influenza virus M2 proton pump, which is conserved through strains and a candidate for universal influenza vaccines. Pigs represent a relevant target of such universal vaccines as pigs can be infected by swine, human and avian strains. We found that cDC1 were the only cell type labeled by XCR1-targeted mCherry upon intradermal injection in pig skin. XCR1-targeted M2e induced higher IgG responses in seronegative and seropositive pigs as compared to non-targeted M2e. The IgG response was less significantly enhanced by CpG than by XCR1 targeting, and CpG did not further increase the response elicited by XCR1 targeting. Monophosphoryl lipid A with neutral liposomes did not have significant effect. Thus altogether M2e-targeting to XCR1 shows promises for a trans-species universal influenza vaccine strategy, possibly avoiding the use of classical adjuvants.
Mots-clé
Adjuvants, Immunologic/administration & dosage, Animals, Antibodies, Viral/blood, Antibody Formation, Chemokines, C/administration & dosage, Chemokines, C/genetics, Chemokines, C/metabolism, Dendritic Cells/immunology, Dendritic Cells/metabolism, Immunoglobulin G/blood, Influenza Vaccines/administration & dosage, Influenza Vaccines/genetics, Influenza Vaccines/immunology, Oligodeoxyribonucleotides/administration & dosage, Receptors, G-Protein-Coupled/metabolism, Recombinant Fusion Proteins/administration & dosage, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/immunology, Skin/immunology, Skin/metabolism, Swine, Viral Matrix Proteins/administration & dosage, Viral Matrix Proteins/genetics, Viral Matrix Proteins/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/04/2019 16:15
Dernière modification de la notice
21/08/2019 6:36
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