Limiting dilution analysis of alloantigen-reactive T lymphocytes. IV. High frequency of cytolytic T lymphocyte precursor cells in MLC blasts separated by velocity sedimentation

Details

Serval ID
serval:BIB_A9A84269563C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Limiting dilution analysis of alloantigen-reactive T lymphocytes. IV. High frequency of cytolytic T lymphocyte precursor cells in MLC blasts separated by velocity sedimentation
Journal
Journal of Immunology
Author(s)
Maryanski  J. L., MacDonald  H. R., Cerottini  J. C.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
01/1980
Volume
124
Number
1
Pages
42-7
Notes
Journal Article --- Old month value: Jan
Abstract
A sensitive limiting dilution microculture system was used to obtain minimal estimates of the frequency of cytolytic T lymphocyte precursor cells (CTL-P) directed against DBA/2 alloantigens, after priming of spleen cells in unidirectional mixed leukocyte cultures (MLC, C57BL/6 anti-DBA/2). The mean CTL-P frequency in day 4 to 5 MLC populations was found to be approximately 50- to 100-fold greater than the frequency in normal spleen, and up to 25% of the cells present in such MLC could be identified operationally as CTL-P. Even higher frequencies (up to 50%) of CTL-P were obtained in a population of large-sized cells separated from day 4 MLC by velocity sedimentation. Furthermore, since a strikingly quantitative correlation was observed between CTL activity and CTL-P frequency in such separated MLC populations, it is likely that mature CTL in MLC are not end cells, but can further proliferate and thus behave operationally as CTL-P.
Keywords
Animals Cell Separation Cells, Cultured Clone Cells/immunology *Cytotoxicity, Immunologic *Isoantigens Lymphocyte Culture Test, Mixed Mice Mice, Inbred C57BL Mice, Inbred DBA T-Lymphocytes/*immunology/radiation effects Time Factors
Pubmed
Web of science
Create date
28/01/2008 11:14
Last modification date
20/08/2019 15:13
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