Efficacy of Newly Isolated and Highly Potent Bacteriophages in a Mouse Model of XDRAB Bacteremia.

Détails

ID Serval
serval:BIB_A99574873386
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Efficacy of Newly Isolated and Highly Potent Bacteriophages in a Mouse Model of XDRAB Bacteremia.
Périodique
Journal of global antimicrobial resistance
Auteur(s)
Leshkasheli L., Kutateladze M., Balarjishvili N., Bolkvadze D., Save J., Oechslin F., Que Y.A., Resch G.
ISSN
2213-7173 (Electronic)
ISSN-L
2213-7165
Statut éditorial
In Press
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Résumé
Bacteremia can be caused by Acinetobacter baumannii with clinical manifestations ranging from transient bacteremia to septic shock. Extensively drug-resistant A. baumannii (XDRAB) strains producing the New Delhi metallo-ß-lactamase, which confers resistance to all ß-lactams including carbapenems, have emerged and infected patients suffer increased mortality, morbidity and length of hospitalization. The lack of new antimicrobials led to a renewed interest into phage therapy, the so-called forgotten cure. Accordingly, we tested new lytic bacteriophages in a Galleria mellonella and a mouse model of XDRAB-induced bacteremia.
Galleria mellonella were challenged with 5.10 <sup>5</sup> CFU of the XDRAB strain FER. Phages vB_AbaM_3054 and vB_AbaM_3090 were administrated alone or in combination 30 min. after bacterial challenge. Saline and imipenem were injected as controls. Mice were challenged i.p. with 6.10 <sup>7</sup> CFU of A. baumannii FER. vB_AbaM_3054 and vB_AbaM_3090 were administrated i.p. alone or in combination 2 h after bacterial challenge. Saline and imipenem were injected as controls. Larvae and mice survival were followed for 7 days and compared with Log-Rank (Mantel-Cox) and Gehan-Breslow-Wilcoxon tests.
Phage-based treatments showed high efficacy in larvae (ca. 100% survival at 80 h) and mice (ca. 100% survival at day 7) compared to the untreated control (0% survival at 48 h and 24 h in larvae and mice, respectively).
The present data reporting efficacy of phage therapy in a mouse model of bacteremia support the development of phage-based drugs to manage infection due to multi-drug resistant A. baumannii and particularly XDRAB.
Mots-clé
A. baumannii, MDRAB, XDRAB, bacteremia, bacteriophage, phage therapy, sepsis
Pubmed
Open Access
Oui
Création de la notice
25/05/2019 12:40
Dernière modification de la notice
21/08/2019 5:34
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