A part of a book
Review on polyhydroxyalkanoate formation in the model plant Arabidopsis thaliana
Title of the book
1996 International Symposium on Bacterial Polyhydroxyalkanoates
NRC Research Press
Eggink G., Steinbüchel A., Poirier Y., Witholt B.
Davos, Switzerland, 18-23 August 1996 program and book of abstracts |c scientific committee Bernhard Witholt ... [et al.] sponsored by The Swiss Federal Institute of Technology, Zürich (ETH-Zuerich) ... [et al.]
In order to explore potential alternatives to the production of polyhydroxyalkanoates (PHAs) in bacteria, the enzymes of Alcaligenes eutrophus involved in the synthesis of polyhydroxybutyrate (PHB) have been expressed in the model plant Arabidopsis thaliana. Following the successful production of low amounts of high molecular weight PHB in plants expressing the acetoacetyl-CoA reductase and the PHB synthase in the cytoplasm of Arabidopsis cell, expression of the PHB pathway in the pastids was achieved by modifying the PHB enzymes with plastid targeting signals. This strategy resulted in a significant increase in the formation of PHB in Arabidopsis, with a maximum of 14% of the leaf dry weight . The increase in PHB production is most likely due to the higher flux in the plastids of acetyl-CoA, the precursor for PHB synthesis. A detailed study of metabolic fluxes in Arabidopsis plants producing high levels of PHB could help to determine the potential problems and limitations of PHB synthesis in Arabidopsis and could be useful for optimising strategies for the production of PHB in crop plants. The knowledge on PHB production could also be used for the production of PHAs other than PHB. Apart from PHB, no other PHAs have been produced in an eukaryotic system. Arabidopsis will therefore be used as a model system for the production in eukaryotes of more complex PHAs, such as poly(hydroxybutyrate-co-hydroxyvylerate) or medium-chain-lenght-PHAs.
polyhydroxybutyrate, polyhydroxyalkanoates, biopolymers, Arabidopsis thaliana, plastics
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