Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy.

Détails

ID Serval
serval:BIB_A92908DCE34A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spatial analysis of erythrocyte membrane fluctuations by digital holographic microscopy.
Périodique
Blood cells, molecules & diseases
Auteur(s)
Rappaz B., Barbul A., Hoffmann A., Boss D., Korenstein R., Depeursinge C., Magistretti P.J., Marquet P.
ISSN
1096-0961[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
42
Numéro
3
Pages
228-32
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Red blood cell (RBC) membrane fluctuations provide important insights into cell states. We present a spatial analysis of red blood cell membrane fluctuations by using digital holographic microscopy (DHM). This interferometric and dye-free technique, possessing nanometric axial and microsecond temporal sensitivities enables to measure cell membrane fluctuations (CMF) on the whole cell surface. DHM acquisition is combined with a model which allows extracting the membrane fluctuation amplitude, while taking into account cell membrane topology. Uneven distribution of CMF amplitudes over the RBC surface is observed, showing maximal values in a ring corresponding to the highest points on the RBC torus as well as in some scattered areas in the inner region of the RBC. CMF amplitudes of 35.9+/-8.9 nm and 4.7+/-0.5 nm (averaged over the cell surface) were determined for normal and ethanol-fixed RBCs, respectively.
Pubmed
Web of science
Création de la notice
27/04/2009 15:01
Dernière modification de la notice
20/08/2019 15:13
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