High-fat diet consumption alters energy metabolism in the mouse hypothalamus.

Détails

ID Serval
serval:BIB_A7BB61E751A8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High-fat diet consumption alters energy metabolism in the mouse hypothalamus.
Périodique
International journal of obesity
Auteur(s)
Lizarbe B., Cherix A., Duarte JMN, Cardinaux J.R., Gruetter R.
ISSN
1476-5497 (Electronic)
ISSN-L
0307-0565
Statut éditorial
Publié
Date de publication
06/2019
Peer-reviewed
Oui
Volume
43
Numéro
6
Pages
1295-1304
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before.
We used <sup>1</sup> H and <sup>13</sup> C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse cohorts at different time points for up to 4 months.
We found that high-fat diet increases significantly the hypothalamic levels of glucose (P < 0.001), osmolytes (P < 0.001), and neurotransmitters (P < 0.05) from 2 months of diet, and alters the rates of metabolic (P < 0.05) and neurotransmission fluxes (P < 0.001), and the contribution of non-glycolytic substrates to hypothalamic metabolism (P < 0.05) after 10 weeks of high-fat feeding.
We report changes that reveal a high-fat diet-induced alteration of hypothalamic metabolism and neurotransmission that is quantifiable by <sup>1</sup> H and <sup>13</sup> C MRS in vivo, and present the first evidence of the extension of the inflammation pathology to a localized metabolic imbalance.
Pubmed
Web of science
Création de la notice
23/10/2018 10:55
Dernière modification de la notice
20/08/2019 16:12
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