Article: article from journal or magazin.
Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.
Cell Communication and Adhesion
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Intercellular Ca(2+) wave propagation between vascular smooth muscle cells (SMCs) is associated with the propagation of contraction along the vessel. Here, we characterize the involvement of gap junctions (GJs) in Ca(2+) wave propagation between SMCs at the cellular level. Gap junctional communication was assessed by the propagation of intercellular Ca(2+) waves and the transfer of Lucifer Yellow in A7r5 cells, primary rat mesenteric SMCs (pSMCs), and 6B5N cells, a clone of A7r5 cells expressing higher connexin43 (Cx43) to Cx40 ratio. Mechanical stimulation induced an intracellular Ca(2+) wave in pSMC and 6B5N cells that propagated to neighboring cells, whereas Ca(2+) waves in A7r5 cells failed to progress to neighboring cells. We demonstrate that Cx43 forms the functional GJs that are involved in mediating intercellular Ca(2+) waves and that co-expression of Cx40 with Cx43, depending on their expression ratio, may interfere with Cx43 GJ formation, thus altering junctional communication.
Animals, Calcium Signaling/drug effects, Carbenoxolone/pharmacology, Cell Communication, Cells, Cultured, Connexin 43/genetics, Connexin 43/metabolism, Connexins/genetics, Connexins/metabolism, Fluorescent Dyes/metabolism, Gap Junctions/drug effects, Gap Junctions/metabolism, Gene Expression, Isoquinolines/metabolism, Male, Mesenteric Arteries/cytology, Myocytes, Smooth Muscle/metabolism, Octanols/pharmacology, Peptides/pharmacology, Primary Cell Culture, Protein Binding, Protein Transport, Rats, Rats, Wistar, Single-Cell Analysis
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