Subnanogram GC/NPD method for the determination of sparteine in biological fluids.
Details
Serval ID
serval:BIB_A6DDF215688E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Subnanogram GC/NPD method for the determination of sparteine in biological fluids.
Journal
Methods and Findings In Experimental and Clinical Pharmacology
ISSN
0379-0355[print], 0379-0355[linking]
Publication state
Published
Issued date
1989
Volume
11
Number
2
Pages
115-118
Language
english
Abstract
A gas chromatographic method is presented for identification and quantification of sparteine in biological fluids using cyclizine as an internal standard. No derivation is necessary and after a single alkaline extraction, GC analysis of sparteine is achieved in less than 8 min. A subnanogram limit of detection is allowed by the use of a nitrogen phosphorous detector (NPD). This method is simple, reproducible, selective, and applicable in both clinical and pharmacokinetic studies.
Keywords
Chromatography, Gas, Humans, Indicators and Reagents, Sparteine/analysis, Sparteine/blood
Pubmed
Create date
15/10/2010 14:00
Last modification date
20/08/2019 16:11