Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.

Details

Ressource 1Download: 17384130_Postprint.pdf (2692.13 [Ko])
State: Public
Version: author
Serval ID
serval:BIB_A6D8869DE0A7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
Journal
American Journal of Physiology. Heart and Circulatory Physiology
Author(s)
Rajesh M., Mukhopadhyay P., Bátkai S., Haskó G., Liaudet L., Drel V.R., Obrosova I.G., Pacher P.
ISSN
0363-6135 (Print)
ISSN-L
0363-6135
Publication state
Published
Issued date
07/2007
Peer-reviewed
Oui
Volume
293
Number
1
Pages
H610-H619
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Intramural
Publication Status: ppublish
Abstract
A nonpsychoactive cannabinoid cannabidiol (CBD) has been shown to exert potent anti-inflammatory and antioxidant effects and has recently been reported to lower the incidence of diabetes in nonobese diabetic mice and to preserve the blood-retinal barrier in experimental diabetes. In this study we have investigated the effects of CBD on high glucose (HG)-induced, mitochondrial superoxide generation, NF-kappaB activation, nitrotyrosine formation, inducible nitric oxide synthase (iNOS) and adhesion molecules ICAM-1 and VCAM-1 expression, monocyte-endothelial adhesion, transendothelial migration of monocytes, and disruption of endothelial barrier function in human coronary artery endothelial cells (HCAECs). HG markedly increased mitochondrial superoxide generation (measured by flow cytometry using MitoSOX), NF-kappaB activation, nitrotyrosine formation, upregulation of iNOS and adhesion molecules ICAM-1 and VCAM-1, transendothelial migration of monocytes, and monocyte-endothelial adhesion in HCAECs. HG also decreased endothelial barrier function measured by increased permeability and diminished expression of vascular endothelial cadherin in HCAECs. Remarkably, all the above mentioned effects of HG were attenuated by CBD pretreatment. Since a disruption of the endothelial function and integrity by HG is a crucial early event underlying the development of various diabetic complications, our results suggest that CBD, which has recently been approved for the treatment of inflammation, pain, and spasticity associated with multiple sclerosis in humans, may have significant therapeutic benefits against diabetic complications and atherosclerosis.
Keywords
Cannabidiol/administration & dosage, Cell Adhesion/drug effects, Cells, Cultured, Cytokines/immunology, Dose-Response Relationship, Drug, Endothelial Cells/drug effects, Endothelial Cells/immunology, Glucose/administration & dosage, Humans, Inflammation Mediators/immunology, Monocytes/drug effects, Monocytes/immunology
Pubmed
Web of science
Create date
24/01/2008 17:01
Last modification date
20/08/2019 15:11
Usage data