A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity

Détails

ID Serval
serval:BIB_A60B42B8B18F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A versatile and rapid assay of radiosensitivity of peripheral blood leukocytes based on DNA and surface-marker assessment of cytotoxicity
Périodique
Radiation Research
Auteur(s)
Crompton  N. E., Ozsahin  M.
ISSN
0033-7587
Statut éditorial
Publié
Date de publication
01/1997
Peer-reviewed
Oui
Volume
147
Numéro
1
Pages
55-60
Notes
Comparative Study
In Vitro
Journal Article --- Old month value: Jan
Résumé
We describe a rapid assay to predict intrinsic radiosensitivity of normal tissues based on the radiation-induced cytotoxic response of a number of types of white blood cells. Twenty-four hours after irradiation, leukocytes were prepared for analysis by flow cytometry. Radiation-induced cytotoxicity was characterized by degradation of internucleosomal DNA, which results in a reduced G1/G0-phase DNA peak. Clear differences in the radiosensitivity of five of six different types of leukocytes were observed. Significant increases in cytotoxicity were observed even at 0.5 Gy, indicating the technique may be useful as a biological dosimeter after radiation accidents. Based on data from 12 healthy blood donors, significant inter-donor differences in radiosensitivity were observed after exposure to 2 or 8 Gy of high-dose-rate X rays. Inter-donor variation was significantly greater than intra-donor variation for both CD4 and CD8 T lymphocytes (P < 0.01), and the technique yielded adequate data to stratify donors based on the radiosensitivities of these two cell types alone, requiring less than 1 ml of blood. The assay is a general assay of early cytotoxic response to radiation-induced damage and should be useful when assaying the response to chemotherapy as well.
Mots-clé
Adult Aged Antigens, CD/blood B-Lymphocytes/radiation effects CD4-Positive T-Lymphocytes/radiation effects CD8-Positive T-Lymphocytes/radiation effects Cell Cycle/radiation effects Cell Survival/radiation effects DNA/blood/*radiation effects Dose-Response Relationship, Radiation Female Flow Cytometry G0 Phase G1 Phase Granulocytes/radiation effects Humans Killer Cells, Natural/radiation effects Leukocytes/immunology/pathology/*radiation effects Male Middle Aged Monocytes/radiation effects X-Rays
Pubmed
Web of science
Création de la notice
24/01/2008 18:15
Dernière modification de la notice
03/03/2018 20:16
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