Article: article from journal or magazin.
Mouse mammary tumour virus related sequences are present in human DNA
Nucleic Acids Research
Comparative Study Journal Article Research Support, Non-U.S. Gov't --- Old month value: Jun 25
MuMTV-related sequences have been identified in the DNA of human breast cancer cells using the Southern transfer technique and hybridisation with cloned MuMTV DNA under conditions in which partially mismatched sequences form stable hybrids. Hybridisation with cloned fragments of the MuMTV genome showed that the gag-pol region shares the most homology (estimated to be greater than 80%) with the human MuMTV-related sequences, however, DNA fragments partially homologous to the MuMTV LTR, gag ad env regions were also detected. Analysis of several human DNA samples suggests that the majority of the human MuMTV-related sequences are genetically transmitted but additional Eco R1 fragments were detected in the DNA of one out of three breast cancer cell lines, MCF7. These sequences are potential probes for the human MuMTV-related retroviral sequences and will allow their possible role in human breast cancer to be evaluated.
Animals Base Sequence Breast Neoplasms/genetics DNA/*analysis DNA, Viral/*analysis Humans Mammary Tumor Virus, Mouse/*genetics Mice Nucleic Acid Hybridization
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