Preconditioning with cromakalim improves long-term myocardial preservation for heart transplantation.

Details

Serval ID
serval:BIB_A5833EBFBB21
Type
Article: article from journal or magazin.
Collection
Publications
Title
Preconditioning with cromakalim improves long-term myocardial preservation for heart transplantation.
Journal
The Annals of thoracic surgery
Author(s)
Kirsch M., Baufreton C., Fernandez C., Brunet S., Pasteau F., Astier A., Loisance D.Y.
ISSN
0003-4975 (Print)
ISSN-L
0003-4975
Publication state
Published
Issued date
08/1998
Peer-reviewed
Oui
Volume
66
Number
2
Pages
417-424
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
Myocardial preservation for heart transplantation relies on hyperkalemic cardiac arrest and hypothermic storage. Our study investigated whether pretreatment with a potassium-channel opener (cromakalim) before prolonged storage in an extracellular fluid improves left ventricular recovery.
Rabbit hearts were submitted to 6-hours' cold storage and assessed on a blood-perfused isolated heart preparation. Hemodynamic recovery, enzyme release (creatine kinase and lactate dehydrogenase), and adenine nucleotide content were determined. Five groups were tested: control (n=6), no ischemia; UW group (n=7), hearts arrested with and stored in University of Wisconsin solution; STH group (n=5), hearts arrested with and stored in St. Thomas' Hospital solution; cromakalim group (n=6), hearts pretreated with cromakalim (30 microg/kg) before arrest with and storage in St. Thomas' Hospital solution; and glibenclamide group (n=5), hearts pretreated with cromakalim followed by glibenclamide (a potassium-channel blocker) before arrest with and storage in St. Thomas' Hospital solution.
Hemodynamic recovery was improved and enzyme release was lower in the UW group than in the STH group. Compared with the STH group, the group pretreated with cromakalim had significantly decreased left ventricular end-diastolic pressures, increased left ventricular developed pressures, increased maximal values of positive and negative rates of rise of left ventricular pressure, and increased time constant of isovolumetric relaxation. Hemodynamic recovery was similar in the UW group and cromakalim groups. Glibenclamide did not abolish the effects of cromakalim. None of the protocols affected myocardial energy stores.
Pretreatment with cromakalim affords additional protection to that provided by cardioplegic arrest and prolonged cold storage using an extracellular solution. The intracellular mechanisms involved remain to be determined.
Keywords
Adenine Nucleotides/analysis, Adenosine, Allopurinol, Animals, Bicarbonates/pharmacology, Calcium Chloride/pharmacology, Cardioplegic Solutions/pharmacology, Coronary Circulation, Creatinine/metabolism, Cromakalim/pharmacology, Glutathione, Glyburide/pharmacology, Heart/physiology, Heart Transplantation, Hemodynamics, Insulin, L-Lactate Dehydrogenase/metabolism, Magnesium/pharmacology, Organ Preservation/methods, Organ Preservation Solutions, Potassium Channels/drug effects, Potassium Chloride/pharmacology, Rabbits, Raffinose, Sodium Chloride/pharmacology
Pubmed
Web of science
Create date
30/03/2019 17:13
Last modification date
20/08/2019 15:10
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