Leptin inhibits directly glucocorticoid secretion by normal human and rat adrenal gland.

Détails

ID Serval
serval:BIB_A5433DF9B2B1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Leptin inhibits directly glucocorticoid secretion by normal human and rat adrenal gland.
Périodique
Endocrinology
Auteur(s)
Pralong F.P., Roduit R., Waeber G., Castillo E., Mosimann F., Thorens B., Gaillard R.C.
ISSN
0013-7227
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
139
Numéro
10
Pages
4264-4268
Langue
anglais
Résumé
Different interactions have been described between glucocorticoids and the product of the ob gene leptin. Leptin can inhibit the activation of the hypothalamo-pituitary-adrenal axis by stressful stimuli, whereas adrenal glucocorticoids stimulate leptin production by the adipocyte. The present study was designed to investigate the potential direct effects of leptin to modulate glucocorticoid production by the adrenal. Human adrenal glands from kidney transplant donors were dissociated, and isolated primary cells were studied in vitro. These cells were preincubated with recombinant leptin (10(-10)-10(-7) M) for 6 or 24 h, and basal or ACTH-stimulated cortisol secretion was subsequently measured. Basal cortisol secretion was unaffected by leptin, but a significant and dose-dependent inhibition of ACTH-stimulated cortisol secretion was observed [down by 29 +/- 0.1% of controls with the highest leptin dose, P < 0.01 vs. CT (unrelated positive control)]. This effect of leptin was also observed in rat primary adrenocortical cells, where leptin inhibited stimulated corticosterone secretion in a dose-dependent manner (down by 46 +/- 0.1% of controls with the highest leptin dose, P < 0.001 vs. CT). These effects of leptin in adrenal cells are likely mediated by the long isoform of the leptin receptor (OB-R), because its transcript was found to be expressed in the adrenal tissue and leptin had no inhibitory effect in adrenal glands obtained from db/db mice. Therefore, leptin inhibits directly stimulated cortisol secretion from human and rat adrenal glands, and this may represent an important mechanism to modulate glucocorticoid levels in various metabolic states.
Mots-clé
Adrenal Glands, Adrenocorticotropic Hormone, Animals, Corticosterone, Dose-Response Relationship, Drug, Female, Humans, Hydrocortisone, Leptin, Proteins, Rats, Rats, Wistar
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:41
Dernière modification de la notice
08/05/2019 23:12
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