Isolation of multipotent neural precursors residing in the cortex of the adult human brain

Détails

ID Serval
serval:BIB_A5313C94B214
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation of multipotent neural precursors residing in the cortex of the adult human brain
Périodique
Experimental Neurology
Auteur(s)
Arsenijevic  Y., Villemure  J. G., Brunet  J. F., Bloch  J. J., Deglon  N., Kostic  C., Zurn  A., Aebischer  P.
ISSN
0014-4886
Statut éditorial
Publié
Date de publication
07/2001
Peer-reviewed
Oui
Volume
170
Numéro
1
Pages
48-62
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
Multipotent precursors able to generate neurons, astrocytes, and oligodendrocytes have previously been isolated from human brain embryos and recently from neurogenic regions of the adult human brains. The isolation of multipotent neural precursors from adult human should open new perspectives to study adult neurogenesis and for brain repair. The present study describes the in vitro isolation from adult human brains of a progenitor responsive to both epidermal and basic fibroblast growth factors that forms spheres as it proliferates. Single spheres derived from various regions of the brain generate in vitro neurons, astrocytes, and oligodendrocytes. The clonal origin of the spheres was revealed by genomic viral insertion using lentiviral vector. Interestingly, this vector appears to be a potent tool for gene transfer into human neural progeny. Ninety-six percent of the spheres investigated were multipotent. Multipotent precursors were isolated from all brain regions studied, including the temporal and the frontal cortex, the amygdala, the hippocampus, and the ventricular zone. This study is the first evidence that primitive precursors such as multipotent precursors exist in the adult human cortex and can reside far from the ventricles. Neurogenesis derived from adult human progenitors differ to murine neurogenesis by the requirement of laminin for oligodendrocyte generation and by the action of basic-fibroblast growth factor and platelet derived growth factor that prevented the formation of oligodendrocytes and neurons. Moreover, the differentiation of human adult precursors seems to differ from fetal ones: adult precursors do not necessitate the removal of mitogen for differentiation. These results indicate that the study of adult multipotent precursors is a new platform to study adult human neurogenesis, potentially generate neural cells for transplantation, and design protocols for in vivo stimulation.
Mots-clé
Adolescent Adult Amygdala/cytology/drug effects Antigens, Differentiation/biosynthesis Brain/*cytology/drug effects Cell Differentiation Cell Division/drug effects Cell Separation Cells, Cultured Cerebral Cortex/cytology/drug effects Child Child, Preschool Clone Cells/cytology Epidermal Growth Factor/pharmacology Extrachromosomal Inheritance Fibroblast Growth Factor 2/pharmacology Humans Infant Middle Aged Neuroglia/cytology/metabolism Neurons/*cytology/metabolism Stem Cells/*cytology/drug effects
Pubmed
Web of science
Création de la notice
06/02/2008 10:44
Dernière modification de la notice
03/03/2018 20:15
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