Ionizing radiation induces early, sustained increases in collagen biosynthesis: a 48-week study in mouse skin and skin fibroblast cultures

Détails

ID Serval
serval:BIB_A525F6E97E4E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ionizing radiation induces early, sustained increases in collagen biosynthesis: a 48-week study in mouse skin and skin fibroblast cultures
Périodique
Radiation Research
Auteur(s)
Panizzon  R. G., Hanson  W. R., Schwartz  D. E., Malkinson  F. D.
ISSN
0033-7587 (Print)
Statut éditorial
Publié
Date de publication
10/1988
Volume
116
Numéro
1
Pages
145-56
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Groups of 10 CF1 female mice, irradiated to the thorax with a dual-head 137Cs gamma-RAY source, received single doses of 0, 5, 10, 15, or 25 Gy. One to forty-eight weeks later collagen synthesis was measured in minced skin specimens incubated in medium containing [3H]proline and then assayed for radioactive hydroxyproline. A progressive, generally dose-dependent increase in collagen biosynthesis, up to 50% above control sites, was found 1, 4, and 12 weeks after radiation exposure. These changes showed further small fluctuations at 12-36 weeks, increasing again at the 48-week interval. At the same times throughout the study fibroblasts were cultured from skin explants. Following the second subculture, these cells were also incubated in medium containing [3H]proline, and collagen synthesis was again determined by [3H]hydroxyproline assay. At all radiation dose levels studied, collagen production increased threefold by 12 weeks postradiation and remained elevated for the 48-week duration of the study. In vitro radiation dose response differences were not observed.
Mots-clé
Animals Cesium Radioisotopes Collagen/*biosynthesis Female Fibroblasts/metabolism/pathology/*radiation effects Fibrosis Gamma Rays Mice Skin/metabolism/pathology/*radiation effects Time Factors
Pubmed
Web of science
Création de la notice
25/01/2008 16:54
Dernière modification de la notice
20/08/2019 15:10
Données d'usage