Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis.

Détails

ID Serval
serval:BIB_A4E7626D1EC3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis.
Périodique
Molecular Microbiology
Auteur(s)
Smits W.K., Bongiorni C., Veening J.W., Hamoen L.W., Kuipers O.P., Perego M.
ISSN
0950-382X (Print)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
2007
Volume
65
Numéro
1
Pages
103-120
Langue
anglais
Résumé
In bacterial differentiation, mechanisms have evolved to limit cells to a single developmental pathway. The establishment of genetic competence in Bacillus subtilis is controlled by a complex regulatory circuit that is highly interconnected with the developmental pathway for spore formation, and the two pathways appear to be mutually exclusive. Here we show by in vitro and in vivo analyses that a member of the Rap family of proteins, RapH, is activated directly by the late competence transcription factor ComK, and is capable of inhibiting both competence and sporulation. Importantly, RapH is the first member of the Rap family that demonstrates dual specificity, by dephosphorylating the Spo0F-P response regulator and inhibiting the DNA-binding activity of ComA. The protein thus acts at the stage where competence is well initiated, and prevents initiation of sporulation in competent cells as well as contributing to the escape from the competent state. A deletion of rapH induces both differentiation pathways and interferes with their temporal separation. Together, these results indicate that RapH is an integral part of a multifactorial regulatory circuit affecting the cell's decision between distinct developmental pathways.
Mots-clé
Bacillus subtilis/cytology, Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Cell Differentiation, Gene Expression Regulation, Bacterial, Microscopy, Fluorescence, Phosphoprotein Phosphatases/genetics, Phosphoprotein Phosphatases/metabolism, Spores, Bacterial/physiology, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Web of science
Création de la notice
11/10/2016 16:26
Dernière modification de la notice
03/03/2018 20:14
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