Detection of specific antigens in the vesicular-arbuscular mycorrhizal fungi Gigaspora margarita and Acaulospora laevis using polyclonal antibodies to soluble spore fractions

Détails

ID Serval
serval:BIB_A481367892C0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of specific antigens in the vesicular-arbuscular mycorrhizal fungi Gigaspora margarita and Acaulospora laevis using polyclonal antibodies to soluble spore fractions
Périodique
Mycological Research
Auteur(s)
Sanders I.R., Ravolanirina F., Gianinazzipearson V., Gianinazzi S., Lemoine M.C.
ISSN
0953-7562
Statut éditorial
Publié
Date de publication
1992
Volume
96
Numéro
6
Pages
477-480
Langue
anglais
Notes
6 JA899 MYCOL RES
Résumé
Polyclonal antibodies raised against soluble extracts of spores of Acaulospora laevis and Gigaspora margarita only cross- reacted in a dot immunobinding assay (DIBA) with heterologous antigens from spores of some of the VAM fungi tested. Major cross-reactivity was between A. laevis and Gig. margarita. Ammonium sulphate precipitation of protein antigens from extracts of these two fungi or their treatment with periodate rendered the activity of antibodies specific indicating that cross-reactions between the fungal extracts in DIBA is probably due to the presence of non-specific glycoconjugates. Analysis of extracts by an indirect enzyme-linked immunosorbent assay (ELISA) gave more specific reactions and immunoblotting of PAGE-separated extracts showed that homologous antisera reacted with different protein bands in the two fungi.
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Création de la notice
24/01/2008 18:37
Dernière modification de la notice
03/03/2018 20:13
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