Variation in allele frequencies at the bg112 locus reveals unequal inheritance of nuclei in a dikaryotic isolate of the fungus Rhizophagus irregularis.

Détails

Ressource 1Télécharger: s00572-018-0834-z.pdf (641.35 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_A47CB11BB965
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Variation in allele frequencies at the bg112 locus reveals unequal inheritance of nuclei in a dikaryotic isolate of the fungus Rhizophagus irregularis.
Périodique
Mycorrhiza
Auteur(s)
Masclaux F.G., Wyss T., Mateus-Gonzalez I.D., Aletti C., Sanders I.R.
ISSN
1432-1890 (Electronic)
ISSN-L
0940-6360
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
28
Numéro
4
Pages
369-377
Langue
anglais
Résumé
The genetic state of the arbuscular mycorrhizal fungus species Rhizophagus irregularis differs among isolates, including both homokaryotic and dikaryotic isolates. Via the production of multi-nucleate axexual spores, siblings of dikaryotic isolates may inherit unequal frequencies of nucleotypes. Using bg112, a microsatellite marker, previous studies revealed that lines deriving from single spores of the dikaryotic R. irregularis isolate C3 differed in their proportions of different alleles. A genomic study of single nuclei of R. irregularis, however, suggested that this marker was a multi-copy locus and that therefore it was inappropriate to study the inheritance of nuclei in dikaryotic isolates. In this study, we first analysed whole genome data of several R. irregularis isolates and demonstrated that bg112 is indeed a single copy locus in these genomes. Thus, the bg112 locus is a suitable marker to study the relative frequency of nucleotypes in R. irregularis. Second, by using amplicon sequencing, we confirmed the existence of one allele of bg112 in two homokaryotic isolates (DAOM197198 and C2) and two alleles in the dikaryotic isolate (C3). Finally, we found that the relative proportions of two bg112 alleles differed significantly among dikaryotic single-spore lines derived from isolate C3, indicating that genetically different nucleotypes are inherited unequally in this dikaryotic R. irregularis isolate.
Mots-clé
Arbuscular mycorrhizal fungi, Nucleotype frequency, Nucleus inheritance, Rhizophagus irregularis, Single-spore lines
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/05/2018 8:54
Dernière modification de la notice
08/05/2019 23:09
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