Oxidation of benzo(a)pyrene by extracellular ligninases of Phanerochaete chrysosporium. Veratryl alcohol and stability of ligninase.

Détails

ID Serval
serval:BIB_A4535BF5A882
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Oxidation of benzo(a)pyrene by extracellular ligninases of Phanerochaete chrysosporium. Veratryl alcohol and stability of ligninase.
Périodique
Journal of Biological Chemistry
Auteur(s)
Haemmerli S.D., Leisola M.S., Sanglard D., Fiechter A.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
05/1986
Peer-reviewed
Oui
Volume
261
Numéro
15
Pages
6900-6903
Langue
anglais
Résumé
Benzo(a)pyrene was oxidized with crude and purified extracellular ligninase preparations from Phanerochaete chrysosporium. Both the crude enzyme and the purified fractions oxidized the substrate to three organic soluble products, namely benzo(a)pyrene 1,6-, 3,6-, and 6,12-quinones. These findings support the recent proposition that lignin-degrading enzymes are peroxidases, mediating oxidation of aromatic compounds via aryl cation radicals. The ligninase which was unstable in the presence of hydrogen peroxide could be stabilized by addition of 3,4-dimethoxy benzyl alcohol to the reaction mixture. The oxidation of benzo(a)pyrene was enhanced in the presence of this alcohol.
Mots-clé
Basidiomycota/enzymology, Benzo(a)pyrene/metabolism, Benzyl Alcohols/pharmacology, Benzyl Compounds/pharmacology, Chromatography, High Pressure Liquid, Drug Stability, Kinetics, Oxidation-Reduction, Oxygenases/isolation & purification, Oxygenases/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 15:40
Dernière modification de la notice
03/03/2018 20:13
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