Evaluation of the Organon-Teknika MICRO-ID LISTERIA system

Détails

ID Serval
serval:BIB_A4448F81FFE3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evaluation of the Organon-Teknika MICRO-ID LISTERIA system
Périodique
Applied and Environmental Microbiology
Auteur(s)
Bannerman  E., Yersin  M. N., Bille  J.
ISSN
0099-2240
Statut éditorial
Publié
Date de publication
06/1992
Peer-reviewed
Oui
Volume
58
Numéro
6
Pages
2011-5
Notes
Comparative Study
Journal Article --- Old month value: Jun
Résumé
The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sources belonging to the seven species currently defined within the genus Listeria. The kit was easy to use and simple to interpret. However, 8 of the 15 tests (i.e., phenylalanine deaminase, hydrogen sulfide, indole, ornithine decarboxylase, lysine decarboxylase, malonate, urease, and o-nitrophenyl-beta-D-galactopyranoside) were considered superfluous for the differentiation of Listeria spp. The CAMP test was indispensable when using the MICRO-ID LISTERIA system, in particular to differentiate CAMP test-positive L. monocytogenes from the nonhemolytic, rhamnose-positive L. innocua. The hemolytic L. seeligeri and L. ivanovii strains and the nonhemolytic, non-rhamnose-acidifying L. welshimeri strains could also be differentiated from one another only on the basis of their CAMP test results. The very few strains of L. grayi and L. murrayi were easily differentiated from the other nonhemolytic species. Catalase-negative cocci should not be tested, because 12 out of 19 catalase-negative strains (all enterococci) in our test were misidentified as Listeria spp. The MICRO-ID LISTERIA system identified strains within 18 to 24 h and is thus less time-consuming than conventional tests. The system could, therefore, be used together with correctly done CAMP tests for the rapid identification of Listeria isolates, especially food and environmental isolates, for which rapid species differentiation is important.
Mots-clé
Animals *Bacteriological Techniques Environmental Microbiology Evaluation Studies as Topic Food Microbiology Humans Listeria/classification/*isolation & purification/metabolism Species Specificity
Pubmed
Web of science
Création de la notice
11/02/2008 13:40
Dernière modification de la notice
03/03/2018 20:13
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