The use of 68Ga-EDTA PET allows detecting progressive decline of renal function in rats.

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Serval ID
serval:BIB_A42CB7293042
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The use of 68Ga-EDTA PET allows detecting progressive decline of renal function in rats.
Journal
American journal of nuclear medicine and molecular imaging
Author(s)
Fontana A.O., Gonzalez Melo M., Allenbach G., Georgantas C., Wang R., Braissant O., Barbey F., Prior J.O., Ballhausen D., Viertl D.
ISSN
2160-8407 (Print)
Publication state
Published
Issued date
2021
Peer-reviewed
Oui
Volume
11
Number
6
Pages
519-528
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Evaluation of glomerular filtration rate is very important in both preclinical and clinical setting, especially in the context of chronic kidney disease. It is typically performed using <sup>51</sup> Cr-EDTA or by imaging with <sup>123</sup> I-Hippuran scintigraphy, which has a significantly lower resolution and sensitivity as compared to PET. <sup>68</sup> Ga-EDTA represents a valid alternative due to its quick availability using a <sup>68</sup> Ge/ <sup>68</sup> Ga generator, while PET/CT enables both imaging of renal function and accurate quantitation of clearance of activity from both plasma and urine. Therefore, we aimed at investigating the use of <sup>68</sup> Ga-EDTA as a preclinical tracer for determining renal function in a knock-in rat model known to present progressive decline of renal function.
<sup>68</sup> Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months.
Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations.
<sup>68</sup> Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment.
Keywords
Glomerular filtration rate, gallium-68 EDTA, positron emission tomography, renal plasma function, rutland-patlak plot
Pubmed
Web of science
Create date
17/01/2022 11:15
Last modification date
20/07/2022 5:38
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