The use of 68Ga-EDTA PET allows detecting progressive decline of renal function in rats.
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Serval ID
serval:BIB_A42CB7293042
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The use of 68Ga-EDTA PET allows detecting progressive decline of renal function in rats.
Journal
American journal of nuclear medicine and molecular imaging
ISSN
2160-8407 (Print)
Publication state
Published
Issued date
2021
Peer-reviewed
Oui
Volume
11
Number
6
Pages
519-528
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Abstract
Evaluation of glomerular filtration rate is very important in both preclinical and clinical setting, especially in the context of chronic kidney disease. It is typically performed using <sup>51</sup> Cr-EDTA or by imaging with <sup>123</sup> I-Hippuran scintigraphy, which has a significantly lower resolution and sensitivity as compared to PET. <sup>68</sup> Ga-EDTA represents a valid alternative due to its quick availability using a <sup>68</sup> Ge/ <sup>68</sup> Ga generator, while PET/CT enables both imaging of renal function and accurate quantitation of clearance of activity from both plasma and urine. Therefore, we aimed at investigating the use of <sup>68</sup> Ga-EDTA as a preclinical tracer for determining renal function in a knock-in rat model known to present progressive decline of renal function.
<sup>68</sup> Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months.
Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations.
<sup>68</sup> Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment.
<sup>68</sup> Ga-EDTA was injected in 23 rats, either wild type (n=10) or knock-in (n=13). By applying a unidirectional, two-compartment model and Rutland-Patlak Plot linear regression analysis, split renal function was determined from the age of 6 weeks to 12 months.
Glomerular filtration ranged from 0.025±0.01 ml/min at 6 weeks to 0.049±0.05 ml/min at 6 months in wild type rats. Glomerular filtration was significantly lower in knock-in rats at 6 and 12 months (P<0.01). No significant difference was observed in renal volumes between knock-in and wild type animals, based on imaging-derived volume calculations.
<sup>68</sup> Ga-EDTA turned out to be a very promising PET/CT tracer for the evaluation of split renal function. This method allowed detection of progressive renal impairment in a knock-in rat model. Additional validation in a human cohort is warranted to further assess clinical utility in both, healthy individuals and patients with renal impairment.
Keywords
Glomerular filtration rate, gallium-68 EDTA, positron emission tomography, renal plasma function, rutland-patlak plot
Pubmed
Web of science
Create date
17/01/2022 11:15
Last modification date
20/07/2022 5:38