Sensitive photonic system to measure oxidative potential of airborne nanoparticles and ROS levels in exhaled air

Détails

Ressource 1Télécharger: BIB_A4206687F497.P001.pdf (1061.99 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_A4206687F497
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Collection
Publications
Titre
Sensitive photonic system to measure oxidative potential of airborne nanoparticles and ROS levels in exhaled air
Titre de la conférence
Eurosensors 2015, XXIX ed., Freiburg, Germany, September 6-9, 2015
Auteur(s)
Laulagnet Alexis, Sauvain Jean-Jacques, Concha-Lozano Nicolas, Riediker Michael, Suarez Guillaume
ISBN
1877-7058
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
120
Série
Procedia Engineering
Pages
632-636
Langue
anglais
Résumé
A photonic system has been developed that enables sensitive quantitative determination of reactive oxygen species (ROS) - mainly hydrogen peroxide (H2O2) - in aerosol samples such as airborne nanoparticles and exhaled air from patients. The detection principle relies on the amplification of the absorbance under multiple scattering conditions due to optical path lengthening [1] and [2]. In this study, the presence of cellulose membrane that acts as random medium into the glass optical cell considerably improved the sensitivity of the detection based on colorimetric FOX assay (FeII/orange xylenol). Despite the loss of assay volume (cellulose occupies 75% of cell volume) the limit of detection is enhanced by one order of magnitude reaching the value of 9 nM (H2O2 equivalents). Spectral analysis is performed automatically with a periodicity of 5 to 15 s, giving rise to real-time ROS measurements. Moreover, the elution of air sample into the collection chamber via a micro-diffuser (impinger) enables quantitative determination of ROS contained in or generated from airborne samples. As proof-of-concept the photonic ROS detection system was used in the determination of both ROS generated from traffic pollution and ROS contained in the exhaled breath as lung inflammation biomarkers.
Mots-clé
Nanoparticles , Biosensing Techniques , Reactive Oxygen Species , Hydrogen Peroxide ,
Open Access
Oui
Création de la notice
28/01/2016 18:09
Dernière modification de la notice
08/05/2019 23:08
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