An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients.

Details

Serval ID
serval:BIB_A3D5DD2E8958
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
An ultra-sensitive impedimetric immunosensor for detection of the serum oncomarker CA-125 in ovarian cancer patients.
Journal
Nanoscale
Author(s)
Johari-Ahar M., Rashidi M.R., Barar J., Aghaie M., Mohammadnejad D., Ramazani A., Karami P., Coukos G., Omidi Y.
ISSN
2040-3372 (Electronic)
ISSN-L
2040-3364
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
7
Number
8
Pages
3768-3779
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Effective treatment of ovarian cancer depends upon the early detection of the malignancy. Here, we report on the development of a new nanostructured immunosensor for early detection of cancer antigen 125 (CA-125). A gold electrode was modified with mercaptopropionic acid (MPA), and then consecutively conjugated with silica coated gold nanoparticles (AuNP@SiO2), CdSe quantum dots (QDs) and anti-CA-125 monoclonal antibody (mAb). The engineered MPA|AuNP@SiO2|QD|mAb immunosensor was characterised using transmission electron microscopy (TEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Successive conjugation of AuNP@SiO2, CdSe QD and anti-CA-125 mAb onto the gold electrode resulted in sensitive detection of CA-125 with a limit of detection (LOD) of 0.0016 U mL(-1) and a linear detection range (LDR) of 0-0.1 U mL(-1). Based on the high sensitivity and specificity of the immunosensor, we propose this highly stable and reproducible biosensor for the early detection of CA-125.
Pubmed
Web of science
Create date
13/03/2015 18:48
Last modification date
20/08/2019 15:09
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