High affinity human IFN-gamma-binding capacity is encoded by a single receptor gene located in proximity to c-ros on human chromosome region 6q16 to 6q22.
Details
Serval ID
serval:BIB_A37426E2BE62
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High affinity human IFN-gamma-binding capacity is encoded by a single receptor gene located in proximity to c-ros on human chromosome region 6q16 to 6q22.
Journal
Journal of Immunology
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
1988
Volume
141
Number
3
Pages
856-860
Language
english
Abstract
We have used human-rodent somatic cell hybrids to investigate the regional localization of the IFN-gamma R gene on human chromosome 6 and studied functional and antigenic characteristics of the expressed IFN-gamma R by Scatchard analyses of 125I-IFN-gamma binding and binding of an anti-receptor mAb (A6C5). The data obtained revealed coordinate expression of IFN-gamma- and A6C5-binding capacity as well as competition in binding to chromosome 6-positive hybrids and normal cells, indicating that the A6C5-defined protein is by itself capable of high affinity IFN-gamma binding and, thus, is likely to constitute the major IFN-gamma R protein of distinct cell types. The receptor gene could be allocated to region 6q16 to 6q22, which also contains the c-ros oncogene. Genetic linkage of the IFN-gamma R gene to an oncogene located in a region of non-random chromosomal aberrations may have a causal relationship to the deregulated IFN-gamma R expression in several malignancies.
Keywords
Antibodies, Monoclonal, Binding Sites, Antibody, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 6, Epitopes/analysis, Genetic Linkage, Humans, Hybrid Cells/analysis, Interferon-gamma/metabolism, Oncogenes, Receptors, Immunologic/genetics, Receptors, Immunologic/immunology, Receptors, Interferon, Tumor Cells, Cultured
Pubmed
Web of science
Create date
28/01/2008 12:36
Last modification date
20/08/2019 16:09