Protein-binding microarrays: probing disease markers at the interface of proteomics and genomics.

Détails

ID Serval
serval:BIB_A3424A57C803
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Protein-binding microarrays: probing disease markers at the interface of proteomics and genomics.
Périodique
Trends in Molecular Medicine
Auteur(s)
Kerschgens J., Egener-Kuhn T., Mermod N.
ISSN
1471-499X[electronic], 1471-4914[linking]
Statut éditorial
Publié
Date de publication
2009
Volume
15
Numéro
8
Pages
352-358
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Publication Status: ppublish
Résumé
DNA-binding proteins mediate a variety of crucial molecular functions, such as transcriptional regulation and chromosome maintenance, replication and repair, which in turn control cell division and differentiation. The roles of these proteins in disease are currently being investigated using microarray-based approaches. However, these assays can be difficult to adapt to routine diagnosis of complex diseases such as cancer. Here, we review promising alternative approaches involving protein-binding microarrays (PBMs) that probe the interaction of proteins from crude cell or tissue extracts with large collections of synthetic or natural DNA sequences. Recent studies have demonstrated the use of these novel PBM approaches to provide rapid and unbiased characterization of DNA-binding proteins as molecular markers of disease, for example cancer progression or infectious diseases.
Mots-clé
Animals, Biological Markers/chemistry, Communicable Diseases/genetics, Communicable Diseases/metabolism, DNA/genetics, DNA/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Genomics, Humans, Neoplasms/genetics, Neoplasms/metabolism, Protein Array Analysis/methods, Protein Binding, Proteomics
Pubmed
Web of science
Création de la notice
07/10/2009 15:11
Dernière modification de la notice
20/08/2019 15:08
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