The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence.

Détails

ID Serval
serval:BIB_A2F19FAD1A25
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The mouse telomerase RNA 5"-end lies just upstream of the telomerase template sequence.
Périodique
Nucleic Acids Research
Auteur(s)
Hinkley C.S., Blasco M.A., Funk W.D., Feng J., Villeponteau B., Greider C.W., Herr W.
ISSN
0305-1048[print], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
01/1998
Volume
26
Numéro
2
Pages
532-536
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Telomerase is a ribonucleoprotein enzyme with an essential RNA component. Embedded within the telomerase RNA is a template sequence for telomere synthesis. We have characterized the structure of the 5' regions of the human and mouse telomerase-RNA genes, and have found a striking difference in the location of the template sequence: Whereas the 5'-end of the human telomerase RNA lies 45 nt from the telomerase-RNA template sequence, the 5'-end of the mouse telomerase RNA lies just 2 nt from the telomerase-RNA template sequence. Analysis of genomic sequences flanking the 5'-end of the human and mouse telomerase RNA-coding sequences reveals similar promoter-element arrangements typical of mRNA-type promoters: a TATA box-like element and an upstream region containing a consensus CCAAT box. This putative promoter structure contrasts with that of the ciliate telomerase-RNA genes whose structure resembles RNA polymerase III U6 small nuclear RNA (snRNA) promoters. These and other comparisons suggest that, during evolution, both the RNA-polymerase specificity of telomerase RNA-gene promoters and, more recently, the position of the template sequence in the telomerase RNA changed.
Mots-clé
Animals, Base Sequence, Consensus Sequence, Humans, Mice, Molecular Sequence Data, Promoter Regions, Genetic, RNA/chemistry, RNA, Messenger/chemistry, Sequence Alignment, TATA Box, Telomerase/chemistry, Templates, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
08/05/2019 23:04
Données d'usage