Large T Antigen-Specific Cytotoxic T Cells Protect Against Dendritic Cell Tumors through Perforin-Mediated Mechanisms Independent of CD4 T Cell Help.

Détails

Ressource 1Télécharger: BIB_A2C5176008F7.P001.pdf (5986.68 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_A2C5176008F7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Large T Antigen-Specific Cytotoxic T Cells Protect Against Dendritic Cell Tumors through Perforin-Mediated Mechanisms Independent of CD4 T Cell Help.
Périodique
Frontiers in Immunology
Auteur(s)
Duval A., Fuertes Marraco S.A., Schwitter D., Leuenberger L., Acha-Orbea H.
ISSN
1664-3224 (Electronic)
ISSN-L
1664-3224
Statut éditorial
Publié
Date de publication
2014
Volume
5
Pages
338
Langue
anglais
Résumé
Our newly generated murine tumor dendritic cell (MuTuDC) lines, generated from tumors developing in transgenic mice expressing the simian virus 40 large T antigen (SV40LgT) and GFP under the DC specific promoter CD11c, reproduce the phenotypic and functional properties of splenic wild type CD8α(+) conventional DCs. They have an immature phenotype with low co-stimulation molecule expression (CD40, CD70, CD80, and CD86) that is upregulated after activation with toll-like receptor ligands. We observed that after transfer into syngeneic C57BL/6 mice, MuTuDC lines were quickly rejected. Tumors grew efficiently in large T transgene-tolerant mice. To investigate the immune response toward the large T antigen that leads to rejection of the MuTuDC lines, they were genetically engineered by lentiviral transduction to express luciferase and tested for the induction of DC tumors after adoptive transfer in various gene deficient recipient mice. Here, we document that the MuTuDC line was rejected in C57BL/6 mice by a CD4 T cell help-independent, perforin-mediated CD8 T cell response to the SV40LgT without pre-activation or co-injection of adjuvants. Using depleting anti-CD8β antibodies, we were able to induce efficient tumor growth in C57BL/6 mice. These results are important for researchers who want to use the MuTuDC lines for in vivo studies.
Mots-clé
CD4 T cells, CD8 T cell, dendritic cell, large T antigen, perforin, tolerance
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/05/2015 13:08
Dernière modification de la notice
20/08/2019 16:08
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