Genomics of the new species Kingella negevensis: diagnostic issues and identification of a locus encoding a RTX toxin.

Détails

Ressource 1Télécharger: 28802585.pdf (758.71 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_A2A9CDA1BE86
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Genomics of the new species Kingella negevensis: diagnostic issues and identification of a locus encoding a RTX toxin.
Périodique
Microbes and infection
Auteur(s)
Opota O., Laurent S., Pillonel T., Léger M., Trachsel S., Prod'hom G., Jaton K., Greub G.
ISSN
1769-714X (Electronic)
ISSN-L
1286-4579
Statut éditorial
Publié
Date de publication
11/2017
Peer-reviewed
Oui
Volume
19
Numéro
11
Pages
546-552
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Kingella kingae, producing the cytotoxic RTX protein, is a causative agent of serious infections in humans such as bacteremia, endocarditis and osteoarticular infection, especially in young children. Recently, Kingella negevensis, a related species, has been isolated from the oral cavity of healthy children. In this study, we report the isolation of K. negevensis strain eburonensis, initially misidentified as K. kingae with MALDI-TOF MS, from a vaginal specimen of a patient suffering of vaginosis. The genome sequencing and analysis of this strain together with comparative genomics of the Kingella genus revealed that K. negevensis possesses a full homolog of the rtx operon of K. kingae involved in the synthesis of the RTX toxin. We report that a K. kingae specific diagnostic PCR, based on the rtxA gene, was positive when tested on K. negevensis strain eburonensis DNA. This cross-amplification, and risk of misidentification, was confirmed by in silico analysis of the target gene sequence. To overcome this major diagnostic issue we developed a duplex real-time PCR to detect and distinguish K. kingae and K. negevensis. In addition to this, the identification of K. negevensis raises a clinical issue in term of pathogenic potential given the production of a RTX hemolysin.
Mots-clé
Bacterial Toxins/genetics, Female, Genomics, Humans, Kingella/genetics, Kingella/isolation & purification, Neisseriaceae Infections/diagnosis, Neisseriaceae Infections/genetics, Neisseriaceae Infections/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Real-Time Polymerase Chain Reaction, Vaginosis, Bacterial/microbiology, Young Adult, Hemolysin proteins, Infection, Kingella kingae, Matrix-assisted laser desorption-ionization mass spectrometry, Real-time polymerase chain reaction
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/09/2017 12:52
Dernière modification de la notice
08/05/2019 23:03
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