Recognition of synthetic polypeptides corresponding to the N- and C-terminal fragments of Plasmodium falciparum Exp-1 by T-cells and plasma from human donors from African endemic areas

Détails

ID Serval
serval:BIB_A26543FF329A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Recognition of synthetic polypeptides corresponding to the N- and C-terminal fragments of Plasmodium falciparum Exp-1 by T-cells and plasma from human donors from African endemic areas
Périodique
Parasite Immunology
Auteur(s)
Meraldi  V., Nebie  I., Moret  R., Cuzin-Ouattara  N., Thiocone  A., Doumbo  O., Esposito  F., Traore  A. S., Corradin  G., Terenzi  S.
ISSN
0141-9838 (Print)
Statut éditorial
Publié
Date de publication
03/2002
Volume
24
Numéro
3
Pages
141-50
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
The present work describes the recognition of three synthetic polypeptides encompassing the N- and C-terminal regions of the transmembrane Exp-1 protein of the parasite Plasmodium falciparum by plasma and peripheral blood mononuclear cells from naturally exposed individuals living in African endemic areas. The three polypeptides comprise the sequences 23-105, 73-162 and 101-162, and overlap at the transmembrane domain (73-105). Thus, they permitted characterization of the immune response specific to the N- and C-terminal domains in an independent fashion. Two different populations were evaluated, one in the village of Safo in Mali and the other in the villages of Somnaway, Kabortenga and Toussouktenga in Burkina Faso. Antibodies to the sequence 73-162 of Pf Exp-1 were found in 70% of adult Mali donors and in all of the donors tested from Burkina Faso. Strikingly, the N-terminal fragment Pf Exp-1 23-105 was only weakly recognized by a few donors. Evaluation of the T-cell response indicated that the peptide Pf Exp-1 23-105 was more potent than Pf Exp-1 73-162 in inducing a proliferative response. A correlation between peptide-specific interferon-gamma and interleukin-6 production and proliferation to peptide Pf Exp-1 23-105 was observed. Further studies are needed to evaluate this molecule as a vaccine candidate.
Mots-clé
Adolescent Adult Africa/epidemiology Animals Antibodies, Protozoan/blood/*immunology Antigens, Protozoan/*chemistry/*immunology Burkina Faso/epidemiology Cells, Cultured Child, Preschool Endemic Diseases Female Humans Malaria, Falciparum/epidemiology/*immunology Male Mali/epidemiology Peptide Fragments/chemistry/immunology Plasmodium falciparum/*immunology T-Lymphocytes/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
03/03/2018 20:09
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