High protein intake reduces intrahepatocellular lipid deposition in humans.

Détails

ID Serval
serval:BIB_A20F35236974
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High protein intake reduces intrahepatocellular lipid deposition in humans.
Périodique
American Journal of Clinical Nutrition
Auteur(s)
Bortolotti M., Kreis R., Debard C., Cariou B., Faeh D., Chetiveaux M., Ith M., Vermathen P., Stefanoni N.,  K.A., Schneiter P., Krempf M., Vidal H., Boesch C., Tappy L.
ISSN
1938-3207[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
90
Numéro
4
Pages
1002-1010
Langue
anglais
Résumé
BACKGROUND: High sugar and fat intakes are known to increase intrahepatocellular lipids (IHCLs) and to cause insulin resistance. High protein intake may facilitate weight loss and improve glucose homeostasis in insulin-resistant patients, but its effects on IHCLs remain unknown. OBJECTIVE: The aim was to assess the effect of high protein intake on high-fat diet-induced IHCL accumulation and insulin sensitivity in healthy young men. DESIGN: Ten volunteers were studied in a crossover design after 4 d of either a hypercaloric high-fat (HF) diet; a hypercaloric high-fat, high-protein (HFHP) diet; or a control, isocaloric (control) diet. IHCLs were measured by (1)H-magnetic resonance spectroscopy, fasting metabolism was measured by indirect calorimetry, insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp, and plasma concentrations were measured by enzyme-linked immunosorbent assay and gas chromatography-mass spectrometry; expression of key lipogenic genes was assessed in subcutaneous adipose tissue biopsy specimens. RESULTS: The HF diet increased IHCLs by 90 +/- 26% and plasma tissue-type plasminogen activator inhibitor-1 (tPAI-1) by 54 +/- 11% (P < 0.02 for both) and inhibited plasma free fatty acids by 26 +/- 11% and beta-hydroxybutyrate by 61 +/- 27% (P < 0.05 for both). The HFHP diet blunted the increase in IHCLs and normalized plasma beta-hydroxybutyrate and tPAI-1 concentrations. Insulin sensitivity was not altered, whereas the expression of sterol regulatory element-binding protein-1c and key lipogenic genes increased with the HF and HFHP diets (P < 0.02). Bile acid concentrations remained unchanged after the HF diet but increased by 50 +/- 24% after the HFHP diet (P = 0.14). CONCLUSIONS: Protein intake significantly blunts the effects of an HF diet on IHCLs and tPAI-1 through effects presumably exerted at the level of the liver. Protein-induced increases in bile acid concentrations may be involved. This trial was registered at www.clinicaltrials.gov as NCT00523562.
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/10/2009 16:10
Dernière modification de la notice
08/05/2019 23:00
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