Evidence that ferritin is UV inducible in human skin: part of a putative defense mechanism

Détails

ID Serval
serval:BIB_A1E1221A2F70
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evidence that ferritin is UV inducible in human skin: part of a putative defense mechanism
Périodique
Journal of Investigative Dermatology
Auteur(s)
Applegate  L. A., Scaletta  C., Panizzon  R., Frenk  E.
ISSN
0022-202X (Print)
Statut éditorial
Publié
Date de publication
07/1998
Volume
111
Numéro
1
Pages
159-63
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
As ferritin has been identified as an important factor in antioxidant defense in cultured human skin cells we evaluated the presence of ferritin in human skin in vivo and the modifications following irradiation with UVA I, UVA I + II, and solar simulating light by immunohistochemical analysis. We report that the putative protective protein ferritin is regularly present in the basal layer of unirradiated epidermis in vivo and that the induction of ferritin was dependent on wavelength and cell type. Following UVA I radiation, ferritin increased both in epidermal and in dermal tissue. The same response occurred, although to a lesser extent, with UVA I + II but did not occur following solar simulating radiation. Quantitative analysis for ferritin in cultured keratinocytes and fibroblasts from seven individuals following each UV spectra were also assessed by enzyme-linked immunosorbent assay. The induction of ferritin by UV was highly dependent on the waveband and cell type. UVA I and UVA I + II radiations induced ferritin expression in dermal fibroblasts up to 260% and 200% over basal levels, respectively. Solar simulating radiation produced only a small induction of approximately 130% over basal ferritin levels in dermal fibroblasts. Ferritin increased in cultured fibroblasts as early as 3 h post-UVA with a peak at 6 h that remained until 48 h; there was no observable qualitative or quantitative increase seen in the undifferentiated cultured epidermal keratinocytes. Our findings indicate that the putative defense system of ferritin exists in human skin in vivo and its induction is dependent on UV spectra and cell type. The increased concentrations of this antioxidant in human skin following acute UV radiation could afford increased protection against subsequent oxidative stress.
Mots-clé
Adult Aged Aged, 80 and over Female Ferritins/analysis/*biosynthesis Humans Immunohistochemistry Male Middle Aged Skin/metabolism/*radiation effects *Ultraviolet Rays
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 16:55
Dernière modification de la notice
20/08/2019 15:07
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