DNA-DNA interaction in thin layer analysed by cryo-electron microscopy

Détails

ID Serval
serval:BIB_A18C90F72148
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
DNA-DNA interaction in thin layer analysed by cryo-electron microscopy
Périodique
Comptes Rendus de l'Academie des Sciences. Serie III, Sciences de la Vie
Auteur(s)
Leforestier  A., Nissen  H. U., Dubochet  J.
ISSN
0764-4469 (Print)
Statut éditorial
Publié
Date de publication
10/1995
Volume
318
Numéro
10
Pages
1015-20
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Thin vitrified films of 146 base pair double-stranded DNA fragments in semi-dilute solutions are observed by cryo-electron microscopy. Depending on the salt conditions of the buffer and the DNA concentration, the molecules form different characteristic arrangements: they are arranged either locally parallel to each other in random overall orientation or packed perpendicular to the film surface, or else are randomly oriented. These arrangements reflect the weak forces acting between the DNA molecules themselves, as well as between the molecules and the thin film surface.
Mots-clé
Buffers DNA/*analysis/metabolism Drug Interactions Microscopy, Electron/*methods Salts/pharmacology
Pubmed
Web of science
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
03/03/2018 20:08
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