Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly.

Détails

ID Serval
serval:BIB_A0EBFEEF4446
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly.
Périodique
Journal of Virology
Auteur(s)
Pendergrast P.S., Morrison D., Tansey W.P., Hernandez N.
ISSN
0022-538X[print], 0022-538X[linking]
Statut éditorial
Publié
Date de publication
08/1996
Volume
70
Numéro
8
Pages
5025-5034
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human immunodeficiency virus type 1 promoter generates two types of RNA molecules, full-length transcripts and short transcripts. Synthesis of the short transcripts depends on the inducer of short transcripts (IST), an element located downstream of the start site. In the presence of the viral activator Tat, the synthesis of full-length transcripts is up-regulated while that of short transcripts is down-regulated. Full-length and short transcripts are probably generated by different types of transcription complexes. The first is IST independent, capable of efficient elongation, and up-regulated by Tat. The second is IST dependent, incapable of efficient elongation, and down-regulated by Tat. We have used an in vivo assay to assess the role of TBP in human immunodeficiency virus type I transcription and to test the effect of mutations in TBP on synthesis of full-length and short transcripts. We find that TBP bound to the TATA box is required for the synthesis of short and full-length transcripts as well as for Tat activation and that both yeast TBP and the carboxy-terminal domain of human TBP can replace full-length human TBP for these processes. Mutations in TBP affect the synthesis of short and full-length transcripts as well as Tat activation similarly, and these effects correlate with the previously described effects of these mutations on binding of TBP to the TBP-associated factor TAFII250 in vitro. Together, these results suggest that if short and full-length transcripts are generated by variant transcription complexes, these complexes use TBP similarly, probably as part of the TFIID complex.
Mots-clé
Carboxylic Acids, DNA-Binding Proteins/genetics, HIV-1/genetics, HIV-1/metabolism, Humans, Mutation, Proteasome Endopeptidase Complex, RNA, Viral/genetics, TATA Box/genetics, Transcription, Genetic
Pubmed
Web of science
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
20/08/2019 16:07
Données d'usage