Article: article from journal or magazin.
A role for LEDGF/p75 in targeting HIV DNA integration
Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't --- Old month value: Dec
HIV DNA integration is favored in active genes, but the underlying mechanism is unclear. Cellular lens epithelium-derived growth factor (LEDGF/p75) binds both chromosomal DNA and HIV integrase, and might therefore direct integration by a tethering interaction. We analyzed HIV integration in cells depleted for LEDGF/p75, and found that integration was (i) less frequent in transcription units, (ii) less frequent in genes regulated by LEDGF/p75 and (iii) more frequent in GC-rich DNA. LEDGF is thus the first example of a cellular protein controlling the location of HIV integration in human cells.
Adaptor Proteins, Signal Transducing/genetics/*metabolism Blotting, Western Cell Line DNA/*metabolism Gene Silencing HIV/*genetics/physiology HIV Integrase/metabolism Humans Microarray Analysis Transcription Factors/genetics/*metabolism Virus Integration/*physiology
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