Expansion and tissue infiltration of an allospecific CD4+CD25+CD45RO+IL-7Ralphahigh cell population in solid organ transplant recipients.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_9F81B1648B01
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expansion and tissue infiltration of an allospecific CD4+CD25+CD45RO+IL-7Ralphahigh cell population in solid organ transplant recipients.
Périodique
Journal of Experimental Medicine
Auteur(s)
Codarri L., Vallotton L., Ciuffreda D., Venetz J.P., Garcia M., Hadaya K., Buhler L., Rotman S., Pascual M., Pantaleo G.
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
204
Numéro
7
Pages
1533-1541
Langue
anglais
Notes
Publication types: Journal Article
Résumé
It has been recently shown (Seddiki, N., B. Santner-Nanan, J. Martinson, J. Zaunders, S. Sasson, A. Landay, M. Solomon, W. Selby, S.I. Alexander, R. Nanan, et al. 2006. J. Exp. Med. 203:1693-1700.) that the expression of interleukin (IL) 7 receptor (R) alpha discriminates between two distinct CD4 T cell populations, both characterized by the expression of CD25, i.e. CD4 regulatory T (T reg) cells and activated CD4 T cells. T reg cells express low levels of IL-7Ralpha, whereas activated CD4 T cells are characterized by the expression of IL-7Ralpha(high). We have investigated the distribution of these two CD4 T cell populations in 36 subjects after liver and kidney transplantation and in 45 healthy subjects. According to a previous study (Demirkiran, A., A. Kok, J. Kwekkeboom, H.J. Metselaar, H.W. Tilanus, and L.J. van der Laan. 2005. Transplant. Proc. 37:1194-1196.), we observed that the T reg CD25(+)CD45RO(+)IL-7Ralpha(low) cell population was reduced in transplant recipients (P < 0.00001). Interestingly, the CD4(+)CD25(+)CD45RO(+)IL-7Ralpha(high) cell population was significantly increased in stable transplant recipients compared with healthy subjects (P < 0.00001), and the expansion of this cell population was even greater in patients with documented humoral chronic rejection compared with stable transplant recipients (P < 0.0001). The expanded CD4(+)CD25(+)CD45RO(+)IL-7Ralpha(high) cell population contained allospecific CD4 T cells and secreted effector cytokines such as tumor necrosis factor alpha and interferon gamma, thus potentially contributing to the mechanisms of chronic rejection. More importantly, CD4(+)IL-7Ralpha(+)and CD25(+)IL-7Ralpha(+) cells were part of the T cell population infiltrating the allograft of patients with a documented diagnosis of chronic humoral rejection. These results indicate that the CD4(+)CD25(+)IL-7Ralpha(+) cell population may represent a valuable, sensitive, and specific marker to monitor allospecific CD4 T cell responses both in blood and in tissues after organ transplantation.
Mots-clé
Antigens, CD, Antigens, CD4, Antigens, CD45, CD4-Positive T-Lymphocytes, Cytokines, Flow Cytometry, Humans, Immunosuppression, Interleukin-2 Receptor alpha Subunit, Kidney Transplantation, Liver Transplantation, Receptors, Interleukin-7, T-Lymphocytes
Pubmed
Web of science
Création de la notice
29/01/2008 13:52
Dernière modification de la notice
20/08/2019 15:05
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