A simple and rapid procedure for the purification of synthetic polypeptides by a combination of affinity chromatography and methionine chemistry.

Détails

ID Serval
serval:BIB_9F431BC0DC4C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A simple and rapid procedure for the purification of synthetic polypeptides by a combination of affinity chromatography and methionine chemistry.
Périodique
Febs Letters
Auteur(s)
Roggero M.A., Servis C., Corradin G.
ISSN
0014-5793[print], 0014-5793[linking]
Statut éditorial
Publié
Date de publication
1997
Volume
408
Numéro
3
Pages
285-288
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Chemical synthesis of bioactive peptides has become a widespread and rapidly growing technique due to automated and efficient protocols for chain assembly. For most applications, the crude synthetic product must be purified to remove residual reactants, failure sequences and chemically modified peptide species. We propose here a method of universal applicability based on immobilized metal ion affinity chromatography, CNBr cleavage and use of reversible Met-sulfoxide protection. With this method we were able to purify to homogeneity in high yield the PbCS 242-310 polypeptide corresponding to the C-terminal region of Plasmodium berghei CS protein.
Mots-clé
Amino Acid Sequence, Animals, Chromatography, Affinity/methods, Indicators and Reagents, Methionine/chemistry, Peptides/chemical synthesis, Peptides/chemistry, Plasmodium berghei, Protozoan Proteins/chemical synthesis, Protozoan Proteins/chemistry, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
08/05/2019 22:51
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