Therapeutic differentiation and maturation of lymphatic vessels after lymph node dissection and transplantation.

Détails

ID Serval
serval:BIB_9F0BADCF602F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Therapeutic differentiation and maturation of lymphatic vessels after lymph node dissection and transplantation.
Périodique
Nature Medicine
Auteur(s)
Tammela T., Saaristo A., Holopainen T., Lyytikkä J., Kotronen A., Pitkonen M., Abo-Ramadan U., Ylä-Herttuala S., Petrova T.V., Alitalo K.
ISSN
1546-170X (Electronic)
ISSN-L
1078-8956
Statut éditorial
Publié
Date de publication
2007
Volume
13
Numéro
12
Pages
1458-1466
Langue
anglais
Résumé
Surgery or radiation therapy of metastatic cancer often damages lymph nodes, leading to secondary lymphedema. Here we show, using a newly established mouse model, that collecting lymphatic vessels can be regenerated and fused to lymph node transplants after lymph node removal. Treatment of lymph node-excised mice with adenovirally delivered vascular endothelial growth factor-C (VEGF-C) or VEGF-D induced robust growth of the lymphatic capillaries, which gradually underwent intrinsic remodeling, differentiation and maturation into functional collecting lymphatic vessels, including the formation of uniform endothelial cell-cell junctions and intraluminal valves. The vessels also reacquired pericyte contacts, which downregulated lymphatic capillary markers during vessel maturation. Growth factor therapy improved the outcome of lymph node transplantation, including functional reconstitution of the immunological barrier against tumor metastasis. These results show that growth factor-induced maturation of lymphatic vessels is possible in adult mice and provide a basis for future therapy of lymphedema.
Mots-clé
Animals, Cell Communication, Cell Differentiation, Gene Expression Regulation, Humans, Intercellular Signaling Peptides and Proteins/metabolism, Lymph Node Excision, Lymph Nodes/transplantation, Lymphatic Vessels/physiology, Lymphedema/immunology, Lymphedema/pathology, Mice, Neoplasm Metastasis, Neoplasms/immunology, Vascular Endothelial Growth Factor C/metabolism, Vascular Endothelial Growth Factor D/metabolism
Pubmed
Web of science
Création de la notice
20/12/2012 16:39
Dernière modification de la notice
03/03/2018 20:02
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